Journal: Frontiers in Oncology

Article Title: Establishment, characterization, and drug screening of low-passage patient individual non-small cell lung cancer in vitro models including the rare pleomorphic subentity

doi: 10.3389/fonc.2023.1089681

Figure Lengend Snippet: Antibodies used for flow cytometry.

Article Snippet: LYPD3 , APC , Sino Biological Europe GmbH, Düsseldorf, Germany , 11836-H08H.

Techniques: Cytometry

Journal: Frontiers in Oncology

Article Title: Establishment, characterization, and drug screening of low-passage patient individual non-small cell lung cancer in vitro models including the rare pleomorphic subentity

doi: 10.3389/fonc.2023.1089681

Figure Lengend Snippet: Flow cytometry. Histogram overlays of unstained controls (dotted lines for all three cell lines) and measurements for HROLu55 (green), HROLu22 (blue), and HROBML01 (red) for the epitopes CD326, PD-L1, EGFR, CD26, LYPD3, DSG3, CCD59, CD27, and CD90 are shown.

Article Snippet: LYPD3 , APC , Sino Biological Europe GmbH, Düsseldorf, Germany , 11836-H08H.

Techniques: Flow Cytometry

Journal: Scientific Reports

Article Title: DFT/TDDFT calculations of geometry optimization, electronic structure and spectral properties of clevudine and telbivudine for treatment of chronic hepatitis B

doi: 10.1038/s41598-024-58599-2

Figure Lengend Snippet: The bond parameters of Clevudine ( 1 ) and Telbivudine ( 2 ) compounds.

Article Snippet: BD (1) C2-H6 , BD*(1) C3-C4 , 4.41 , 1.02 , 0.060.

Techniques:

Journal: Scientific Reports

Article Title: DFT/TDDFT calculations of geometry optimization, electronic structure and spectral properties of clevudine and telbivudine for treatment of chronic hepatitis B

doi: 10.1038/s41598-024-58599-2

Figure Lengend Snippet: Significantly calculated donor–acceptor interactions in Clevudine at B3LYP/6-311++G (d, p) level of theory.

Article Snippet: BD (1) C2-H6 , BD*(1) C3-C4 , 4.41 , 1.02 , 0.060.

Techniques:

Journal: Scientific Reports

Article Title: DFT/TDDFT calculations of geometry optimization, electronic structure and spectral properties of clevudine and telbivudine for treatment of chronic hepatitis B

doi: 10.1038/s41598-024-58599-2

Figure Lengend Snippet: Significantly calculated donor–acceptor interactions in Telbivudine at B3LYP/6-311++G (d, p) level of theory.

Article Snippet: BD (1) C2-H6 , BD*(1) C3-C4 , 4.41 , 1.02 , 0.060.

Techniques:

Journal: Cell death discovery

Article Title: Single-cell trajectory analysis reveals a CD9 positive state to contribute to exit from stem cell-like and embryonic diapause states and transit to drug-resistant states.

doi: 10.1038/s41420-023-01586-9

Figure Lengend Snippet: Fig. 4 CD9 expression is increased in transitional cluster 9 and regulates JQ1 resistance in SUM149. A Likelihood of cells remaining in the SCLED cluster based on CD9 expression. B CD9 levels of individual cells are mapped on the graph in Fig. 1A. C Scatter plot shows Pearson correlation of average CD9 expression and cluster 9 signature in each dataset in Fig. 3. D Violin plot shows indicated motif activity of CD9 high cluster and CD9 low cluster in K562 and MDAMB468 cells. E Kaplan–Meier curve of relapse-free survival in patients with basal-like breast cancer (n = 65 or n = 165) based on low or high CD9 protein split on median level. Significance is determined by log-rank test. F Changes of in vitro stemness score of SUM149 transfected with shCON or shCD9-1 lentivirus treated with JQ1 (100 nM) for 96 h, 8 days and 10 days. In vitro stemness markers included SETD6, NANOG, POU5F1, MALAT1, and NEAT1 were assessed by RT-PCR and in vitro stemness score calculated as un weighted sum of relative fold change of each gene compared to shCON cells treated with DMSO and shCD9 cells treated with DMSO, respectively. Data represent SEM of three replicates. G Changes of in vitro stemness score of SUM149 treated with JQ1 (100 nM), anti- CD9 and combination for 96 h, 8 days and 10 days. H Cell viability assessed by prestoblue of SUM149 transfected with shCON, shCD9-1, shCD9-2, and shCD9-3 lentivirus and treated with indicated doses of JQ1 for 96 h. I Scatter plot of correlation of cell growth assessed by prestoblue and CD9 expression (left). Scatter plot of cell sensitivity to JQ1 and CD9 expression (right).

Article Snippet: Antibodies used for cytometry were CD9 (C-4, Santa Cruz Biotechnology, #J0819), along with Calcein violet AM (Invitrogen, #65-0854-39), Propidium Iodide (PI) (Invitrogen, 00-6990-50).

Techniques: Expressing, Activity Assay, In Vitro, Transfection, Reverse Transcription Polymerase Chain Reaction

Journal: Cell death discovery

Article Title: Single-cell trajectory analysis reveals a CD9 positive state to contribute to exit from stem cell-like and embryonic diapause states and transit to drug-resistant states.

doi: 10.1038/s41420-023-01586-9

Figure Lengend Snippet: Fig. 5 CD9 contributes to resistance to multiple drugs. A Representative light microscopy images of 3D cultured cell lines treated with indicated drug for 96 h. (JQ1 = 100 nM, MG132 = 400 nM, mTORi = 200 nM, HSP70i = 10 μM, HSP90i = 8 nM, cDDP=300 nM, CHK1i = 1 nM, WEE1i = 60 nM, PARPi=600 nM, Tram=500 nM, CDK4/6i = 1 μM). Data are representative of three replicates. Scale bars: 200 μm. B Bar charts of colony numbers with indicated diameter with indicated treatments in A. C Scatter plots of Pearson correlation of CD9 expression and indicated drug sensitivity in cancer cell lines from CTRPv2. Each dot presents a cell line.

Article Snippet: Antibodies used for cytometry were CD9 (C-4, Santa Cruz Biotechnology, #J0819), along with Calcein violet AM (Invitrogen, #65-0854-39), Propidium Iodide (PI) (Invitrogen, 00-6990-50).

Techniques: Light Microscopy, Cell Culture, Expressing

Journal: Cells

Article Title: Arachidonic Acid Cascade and Eicosanoid Production Are Elevated While LTC4 Synthase Modulates the Lipidomics Profile in the Brain of the HIVgp120-Transgenic Mouse Model of NeuroHIV

doi: 10.3390/cells11132123

Figure Lengend Snippet: Quantification via LC-MS/MS of select eicosanoids in the brain extracts of wildtype (WT) and HIVgp120tg mice. COX pathway products outlined in blue ( A – F ), CYP/8-,12-,15-LOX products are outlined in green ( G – I ) and 5-LOX products are outlined in orange ( J – L ). The source material for the biosynthesis of eicosanoids, AA ( M ), and the total normalized values of all measured eicosanoid derivatives ( N ) are also shown. Values in graphs are mean ± s.e.m.; n = 12 (6 males and 6 females) per genotype; statistical analysis was performed using an unpaired t -test, * p ≤ 0.05, ** p ≤ 0.01 for difference to WT control. Individual data points are represented in the graphs by an ⚪ symbol. Abbreviations: COX, cyclooxygenase; CYP, cytochrome P450; LOX, lipoxygenase; AA, arachidonic acid; PG, prostaglandin; TX, thromboxane; HHT, hydroxyheptadecatrenoic acid; HETE, hydroxyeicosatetraenoic acid; LT, leukotriene/cysteinyl leukotriene (LTC4, LTD4).

Article Snippet: The samples as well as a freshly prepared standard curve consisting of Leukotriene C4, D4, E4 (Cayman 20210, 20310, 20410) were diluted upon loading so that the final concentration of methanol was between 10 and 15% of the total volume.

Techniques: Liquid Chromatography with Mass Spectroscopy, Control